L Signaling Technology), and anti--tubulin mouse monoclonal antibody (Sigma-Aldrich) were used as primary antibodies for detection of the specific proteins.Statistical analysisStatistical analyses were performed with JMP Pro ver. 11.0 (SAS Institute, Cary, NC, USA). The differences between two groups were analyzed by chi-square test, Fisher's exact test, Student's t test or Mann-Whitney Utest, dep